期刊
ACS SYNTHETIC BIOLOGY
卷 3, 期 8, 页码 515-524出版社
AMER CHEMICAL SOC
DOI: 10.1021/sb4001728
关键词
laboratory automation; microfluidics; liquid-handling robot; standardization; DNA assembly; Kunkel DNA mutagenesis
资金
- Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy [DE-AC02-05CH11231]
- Department of Energy, ARPA-E Electrofuels Program [DE-0000206-1577]
- National Science Foundation Graduate Research Fellowship Program [DGE 1106400]
- Berkeley Laboratory Directed Research and Development Program
- TeselaGen Biotechnology, Inc. [WF009700]
To enable protocol standardization, sharing, and efficient implementation across laboratory automation platforms, we have further developed the PR-PR open-source high-level biology-friendly robot programming language as a cross-platform laboratory automation system. Beyond liquid-handling robotics, PR-PR now supports microfluidic and microscopy platforms, as well as protocol translation into human languages, such as English. While the same set of basic PR-PR commands and features are available for each supported platform, the underlying optimization and translation modules vary from platform to platform. Here, we describe these further developments to PR-PR, and demonstrate the experimental implementation and validation of PR-PR protocols for combinatorial modified Golden Gate DNA assembly across liquid-handling robotic, microfluidic, and manual platforms. To further test PR-PR cross-platform performance, we then implement and assess PR-PR protocols for Kunkel DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic and manual platforms.
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