期刊
ACS SYNTHETIC BIOLOGY
卷 3, 期 12, 页码 929-931出版社
AMER CHEMICAL SOC
DOI: 10.1021/sb500036q
关键词
CRISPR/Cas9; synthetic biology; synthetic gene regulation; horizontal gene transfer; conjugation
资金
- National Institutes of Health [P50 GM081879]
- Howard Hughes Medical Institute
- California Institutes for Quantitative Biosciences at UCSF
- NSF SynBERC [EEC-0540879]
- NIH Office of the Director (OD)
- National Institute of Dental & Craniofacial Research (NIDCR)
- NIH DPS [OD017887]
In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. Here, we use conjugation, a natural DNA horizontal transfer process among bacterial species, to deliver an engineered CRISPR interference (CRISPRi) system for targeting specific genes in recipient Escherichia coli cells. We show that delivery of the CRISPRi system is successful and can specifically repress a reporter gene in recipient cells, thereby establishing a new tool for gene regulation across bacterial cells and potentially for bacterial population control.
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