期刊
ACS SYNTHETIC BIOLOGY
卷 2, 期 9, 页码 537-541出版社
AMER CHEMICAL SOC
DOI: 10.1021/sb400021j
关键词
genome engineering; chromosomal integration; DNA assembly; genetic parts; genetic engineering clonetegration
资金
- China Scholarship Council
- U.S. NSF Synthetic Biology Engineering Research Center (SynBERC)
- Human Frontiers Science [RGP051]
- Australian Research Council [DP110100824, DP11010470]
- William H Elliott Biochemistry Fellowship
We describe clonetegration, a method for integrating DNA into prokaryotic chromosomes that 'approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of singles or multiple DNA fragments. Additionally, donetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
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