期刊
ACS SYNTHETIC BIOLOGY
卷 2, 期 10, 页码 568-575出版社
AMER CHEMICAL SOC
DOI: 10.1021/sb4000433
关键词
quorum sensing; cell-cell communication; promoter engineering; gene expression; acyl-homoserine lactone
资金
- National Science Foundation [MCB-1055676]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1055676] Funding Source: National Science Foundation
Quorum sensing (QS) systems enable bacteria to coordinate their behavior as a function of local population density and are often used in synthetic systems that require cell-cell communication. We have engineered the esaR promoter, P-esaR, which is repressed by the QS regulator EsaR EsaR-dependent gene expression from P-esaR is induced by 3-oxo-hexanoyl-homoserine lactone (3OC6HSL). Here, we report a set of modified P-esaR promoters that contain a second EsaR binding site. We observed changes in gene expression levels, regulatory range, 3OC6HSL sensitivity, and the regulatory role of EsaR that are dependent on the position of the second binding site. Combining the new promoters with endogenous 3OC6HSL production led to QS-dependent systems that exhibit a range of expression levels and timing. These promoters represent a new set of tools for modulating QS-dependent gene expression and may be used to tune the regulation of multiple genes in response to a single QS signal.
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