期刊
ACS MACRO LETTERS
卷 7, 期 9, 页码 1105-1110出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsmacrolett.8b00462
关键词
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资金
- National Science Foundation [DMR-1410223, DMR-1253906]
- Pew Charitable Trusts [00026178]
- Delaware COBRE programs
- National Institute of General Medicine Sciences from the National Institutes of Health [NIGMS P20GM104316, 5 P30 GM110758-02]
Synthetic hydrogels have been widely adopted as well-defined matrices for three-dimensional (3D) cell culture, with increasing interest in systems that enable the co-culture of multiple cell types for probing both cell-matrix and cell-cell interactions in studies of tissue regeneration and disease. We hypothesized that the unique dynamic covalent chemistry of self healing hydrogels could be harnessed for not only the encapsulation and culture of human cells but also the subsequent construction of layered hydrogels for 3D co-cultures. To test this, we formed hydrogels using boronic acid-functionalized polymers and demonstrated their self-healing in the presence of physiologically relevant cell culture media. Two model human cell lines, MDA-MB-231 breast cancer cells and CCL151 pulmonary fibroblasts, were encapsulated within these dynamic materials, and good viability was observed over time. Finally, self-healing of cut hydrogel blocks laden with these different cell types was used to create layered hydrogels for the generation of a dynamic co-culture system. This work demonstrates the utility of self-healing materials for multidimensional cultures and establishes approaches broadly useful for a variety of biological applications.
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