期刊
ACS MACRO LETTERS
卷 3, 期 8, 页码 727-731出版社
AMER CHEMICAL SOC
DOI: 10.1021/mz5002993
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资金
- NSF [DMR1206310]
- NIH (NIDCR) [R01 DE022969]
- R W. Gore Fellowship Program
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1229234] Funding Source: National Science Foundation
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1206310] Funding Source: National Science Foundation
Described herein is interfacial bioorthogonal cross-linking, the use of bioorthogonal chemistry to create and pattern biomaterials through diffiision-controlled gelation at the liquid-gel interface. The basis is a rapid (k(2) 284000 M-1 s(-1)) reaction between strained trans-cyclooctene (TCO) and tetrazine (Tz) derivatives. Syringe delivery of Tz-functionalized hyaluronic acid (HA-Tz) to a bath of bis-TCO crosslinker instantly creates microspheres with a cross-linked shell through which bis-TCO diffuses freely to introduce further cross-linking at the interface. Tags can be introduced with 3D resolution without external triggers or templates. Water-filled hydrogel channels were prepared by simply reversing the order of addition. Prostate cancer cells encapsulated in the microspheres have 99% viability, proliferate readily, and form aggregated clusters. This process is projected to be useful in the fabrication of cell-instructive matrices for in vitro tissue models.
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