期刊
THERANOSTICS
卷 2, 期 6, 页码 618-628出版社
IVYSPRING INT PUBL
DOI: 10.7150/thno.4561
关键词
murine embryonic stem cells; gastric cancer cells; target imaging; migration; chemotaxis
资金
- National Key Basic Research Program (973 Project) [2010CB933901]
- Important National Science & Technology Specific Projects [2009ZX10004-311]
- National Natural Scientific Fund [31100717]
- New Century Excellent Talent of Ministry of Education of China [NCET-08-0350]
- Special project for nanotechnology from Shanghai [1052nm04100]
- Shanghai Jiao Tong University Innovation Fund for Postgraduates [AE340201]
- Shanghai Jiao Tong University Investment Fund for Excellent Doctoral Dissertation
Embryonic stem (ES) cells have great potential in applications such as disease modeling, pharmacological screening and stem cell therapies. Up to date, there is no related report on the use of ES cells as tracking and contrast reagents of cancer cells in vivo. Herein we report that DiR-labeled murine ES cells can recognize and target gastric cancer cells in vivo. DiR-labeled murine ES (mES) cells (5x10(6)) were intravenously injected into gastric tumor-bearing mice. The biodistribution of DiR-labeled mES cells was monitored by IVIS imaging within 24 h. Major organs were harvested and analyzed by immunofluorescence staining and Western blotting. Chemotaxis assay was employed to investigate the chemotaxis of ES cells tracking cancer cells. Fluorescent imaging results showed that DiR-labeled mES cells targeted gastric cancer tissue in vivo as early as 10 min post-injection, reaching a peak at 2h post-injection. Immunofluorescence staining and Western blotting results showed gastric cancer tissues specifically expressed SSEA-1. In vitro migration tests confirmed that mES cells actively moved to test sites with different concentration of CXCL12 in a dose-dependent manner. In conclusion, DiR-labeled mES cells may be used for gastric cancer targeted imaging in vivo, and have great potential in applications such as identifying and imaging of early gastric cancer in near future.
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