期刊
THERANOSTICS
卷 2, 期 12, 页码 1160-1173出版社
IVYSPRING INT PUBL
DOI: 10.7150/thno.4492
关键词
non viral gene delivery; gold nanorods; two photon imaging; combinatorial synthesis; polymer library
资金
- National Science Foundation [CBET-082198]
- National Institutes of Health [1R01GM093229-01A1, 5R21CA143505-02]
- Arizona Biomedical Research Commission (ABRC) [1009]
- Science Foundation of Arizona
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0829128] Funding Source: National Science Foundation
A focused library of twenty-one cationic poly(amino ethers) was synthesized following ring-opening polymerization of two diglycidyl ethers by different oligoamines. The polymers were screened in parallel for plasmid DNA (pDNA) delivery, and transgene expression efficacies of individual polymers were compared to those of 25 kDa polyethylenimine (PEI), a current standard for polymer-mediated transgene delivery. Seven lead polymers that demonstrated higher transgene expression than PEI in pancreatic and prostate cancer cells lines were identified from the screen. All seven lead polymers showed highest transgene expression at a polymer: pDNA weight ratio of 5:1 in the MIA PaCa-2 pancreatic cancer cell line. Among the conditions studied, transgene expression efficacy correlated with minimal polymer cytotoxicity but not polyplex sizes. In addition, this study indicated that methylene spacing between amine centers in the monomers, amine content, and molecular weight of the polymers are all significant factors and should be considered when designing polymers for transgene delivery. A lead effective polymer was employed for coating gold nanorods, leading to theranostic nanoassemblies that possess combined transgene delivery and optical imaging capabilities, leading to potential theranostic systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据