期刊
SCIENTIFIC REPORTS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-018-33107-5
关键词
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资金
- Program for research and mobility support of starting researchers of the Czech Academy of Sciences [MSM200311602]
- Finnish Society of Sciences and Letters
- Faculty of Science at the University of Helsinki
- CHEMS doctoral school
- [RVO: 68081715]
Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (T-m) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the T-m of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the T-m of the lipid. The results show that, when the system temperature approaches the T-m, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 degrees C, 41.0 degrees C, and 55.5 degrees C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical T-m values, i.e., 24 degrees C, 41.4 degrees C, and 55 degrees C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the T-m of phospholipids.
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