期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-15844-1
关键词
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资金
- Life Imaging Center (LIC) of the University of Freiburg
- German Research Foundation (DFG) [CRC 1140, CRC 992]
- Heisenberg program
- European Research Council (ERC)
- H2020-IMI2 consortium BEAt-DKD [115974]
- BMBF STOP-FSGS [01GM1518C]
- MSD Life Science Foundation
- Public Interest Incorporated Foundation
- Uehara Memorial Foundation
- Alexander von Humboldt Foundation
- Deutsche Forschungsgemeinschaft [SCHI 871/5, SCHI 871/8, SCHI 871/9, SCHI 871/11, INST 39/900-1, SFB850]
- Excellence Initiative of the German Federal and State Governments (BIOSS) [EXC 294]
- Excellence Initiative of the German Federal and State Governments (BIOSS)
- Freiburg Institute for Advanced Studies (FRIAS)
- German Society of Nephrology DGFN
- lse Kroner Fresenius Stiftung, NAKSYS
- Berta-Ottenstein Programme, Faculty of Medicine, University of Freiburg
- [HU 1016/8-1]
- Grants-in-Aid for Scientific Research [15K19511] Funding Source: KAKEN
Podocytes are highly specialized epithelial cells essentially required to establish and maintain the kidney filtration barrier. Due to their complex cellular architecture these cells rely on an elaborated cytoskeletal apparatus providing plasticity as well as adaptive adhesion properties to withstand significant physical filtration forces. However, our knowledge about podocyte specific components of the cytoskeletal machinery is still incomplete. Employing cross-analysis of various quantitative omics-data sets we identify the WD40-domain containing protein CORO2B as a podocyte enriched protein. Furthermore, we demonstrate the distinct localization pattern of CORO2B to the ventral actin cytoskeleton serving as a physical linkage module to cell-matrix adhesion sites. Analysis of a novel Coro2b knockout mouse revealed that CORO2B modulates stress response of podocytes in an experimental nephropathy model. Using quantitative focal adhesome proteomics we identify the recruitment of CFL1 via CORO2B to focal adhesions as an underlying mechanism. Thus, we describe CORO2B as a novel podocyte enriched protein influencing cytoskeletal plasticity and stress adaptation.
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