期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-11533-1
关键词
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资金
- EPSRC Centre for Innovative Manufacturing in Emergent Macromolecular Therapies
- Aston University
- BBSRC [BB/L000997/1]
- European Research Area initiative on industrial biotechnology (ERA-IB)
- BBSRC [BB/L000997/1] Funding Source: UKRI
- EPSRC [EP/P006485/1] Funding Source: UKRI
Liposomes are lipid based bilayer vesicles that can encapsulate, deliver and release low-soluble drugs and small molecules to a specific target site in the body. They are currently exploited in several nanomedicine formulations. However, their development and application is still limited by expensive and time-consuming process development and production methods. Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are needed. We present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production. The system was evaluated for a range of small vesicles (below 300 nm) varying in lipid composition, size and charge; it offers effective and rapid nanomedicine purification with high lipid recovery (> 98%) combined with effective removal of non-entrapped drug (propofol > 95% reduction of non-entrapped drug present) or protein (ovalbumin > 90% reduction of OVA present) and organic solvent (ethanol > 95% reduction) in less than 4 minutes. The key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.
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