Nogo receptor 1 regulates Caspr distribution at axo-glial units in the central nervous system

Axo-glial units are highly organised microstructures propagating saltatory conduction and are disrupted during multiple sclerosis (MS). Nogo receptor 1 (NgR1) has been suggested to govern axonal damage during the progression of disease in the MS-like mouse model, experimental autoimmune encephalomyelitis (EAE). Here we have identified that adult ngr1(-/-) mice, previously used in EAE and spinal cord injury experime(n)ts, display elongated paranodes, and nodes of Ranvier. Unstructured paranodal regions in ngr1(-/-) mice are matched with more distributed expression pattern of Caspr. Compound action potentials of optic nerves and spinal cords from naive ngr1(-/-) mice are delayed and reduced. Molecular interaction studies revealed enhanced Caspr cleavage. Our data suggest that NgR1 may regulate axo-myelin ultrastructure through Caspr-mediated adhesion, regulating the electrophysiological signature of myelinated axons of central nervous system (CNS).