期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-03226-6
关键词
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资金
- National Basic Research Program of China [2013CB530805, 2014CBA02005]
- National Key Research and Development Program of China [2016 YFC 1306300]
- National Natural Science Foundation of China [31200614, 31400669]
- Beijing Natural Science Foundation [5132028, 7173264, 7172076]
- Fundamental Research Funds for the Central Universities [2015KJJCB21]
- Beijing Normal University [11100704]
- Biologic Medicine Information Center of China, National Scientific Data Sharing Platform for Population and Health
Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of the urinary proteome will provide a basic understanding of urinary proteins. In this paper, we present an in-depth analysis of the urinary proteome based on different separation strategies, including direct one dimensional liquid chromatography-tandem mass spectrometry (LC/MS/MS), two dimensional LC/MS/MS, and gel-eluted liquid fraction entrapment electrophoresis/liquid-phase isoelectric focusing followed by two dimensional LC/MS/MS. A total of 6085 proteins were identified in healthy urine, of which 2001 were not reported in previous studies and the concentrations of 2571 proteins were estimated (spanning a magnitude of 106) with an intensity-based absolute quantification algorithm. The urinary proteins were annotated by their tissue distribution. Detailed information can be accessed at the Human Urine Proteome Database (www.urimarker.com/urine).
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