4.7 Article

The neuronal K+ Cl- co-transporter 2 (Slc12a5) modulates insulin secretion

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SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -

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NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-01814-0

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  1. King Saud University (Saudi Arabia)
  2. US-Brazil Higher Education Consortia Program FIPSE/CAPES Exchange
  3. WSU Research Incentive Fund [226132]
  4. American Diabetes Association [1-17-IBS-258]
  5. NIH [R01DK097829, R01AI114804]
  6. [P30DK017047]

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Intracellular chloride concentration ([Cl-](i)) in pancreatic beta-cells is kept above electrochemical equilibrium due to the predominant functional presence of Cl-loaders such as the Na+ K+ 2Cl(-) cotransporter 1 (Slc12a2) over Cl-extruders of unidentified nature. Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitro functional assays, we establish that the neuronspecific K+ Cl- co-transporter 2 (KCC2, Slc12a5) is expressed in several endocrine cells of the pancreatic islet, including glucagon secreting alpha-cells, but particularly in insulin-secreting beta-cells, where we provide evidence for its role in the insulin secretory response. Three KCC2 splice variants were identified: the formerly described KCC2a and KCC2b along with a novel one lacking exon 25 (KCC2a-S25). This new variant is undetectable in brain or spinal cord, the only and most abundant known sources of KCC2. Inhibition of KCC2 activity in clonal MIN6 beta-cells increases basal and glucose-stimulated insulin secretion and Ca2+ uptake in the presence of glibenclamide, an inhibitor of the ATP-dependent potassium (K-ATP)-channels, thus suggesting a possible mechanism underlying KCC2-dependent insulin release. We propose that the long-time considered neuron-specific KCC2 co-transporter is expressed in pancreatic islet beta-cells where it modulates Ca2+-dependent insulin secretion.

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