期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep39296
关键词
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资金
- Ministry of Education, Culture, Sport, Science, and Technology of Japan [25460970, 23790761]
- Ministry of Health Labour and Welfare
- Grants-in-Aid for Scientific Research [23790761, 25460970] Funding Source: KAKEN
Myeloid-derived suppressor cells (MDSCs) could have important roles in immune regulation, and MDSCs can be induced in patients with various malignant tumors. The immune-suppressive functions of MDSCs in hepatocellular carcinoma (HCC) patients have not been clarified. Therefore, we tried to analyze the biological significance of MDSCs in HCC patients. We quantified PD-L1(+) MDSCs of HCC patients in various conditions by using multi-color flow cytometry analysis. PBMCs from HCC patients contained significantly higher percentages of PD-L1(+) MDSCs in comparison to those from healthy subjects (p < 0.001). The percentages of PD-L1(+) MDSCs were reduced by curative treatment for HCC (p < 0.05), and the percentages of PD-L1(+) MDSCs before treatment were inversely correlated with disease-free survival time. After we cocultivated PBMCs and several liver cancer cell lines in a transwell coculture system, the percentages of PD-L1(+) MDSCs were significantly increased compared with control (p < 0.05). The expression of M-CSF and VEGFA was higher in the cell lines that strongly induced PDL1(+) MDSCs. Peripheral blood from HCC patients had significantly higher percentages of PD-L1(+) MDSCs in comparison to those of healthy subjects, and the percentages of PD-L1(+) MDSCs were reduced by HCC treatment, suggesting that we might use PD-L1(+) MDSCs as a new biomarker of HCC.
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