期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep24526
关键词
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资金
- Agence Nationale de la Recherche
- EC Joint Programme on Neurodegenerative Diseases [JPND-NeuTARGETs-ANR-14-JPCD-0002-02]
- Centre National de la Recherche Scientifique, France Parkinson [113344]
- Fondation de France [2015-00060936]
- Fondation Bettencourt-Schueller
The deposition of fibrillar alpha-synuclein (alpha-syn) within inclusions (Lewy bodies and Lewy neurites) in neurons and glial cells is a hallmark of synucleinopathies. alpha-syn populates a variety of assemblies ranging from prefibrillar oligomeric species to fibrils whose specific contribution to neurodegeneration is still unclear. Here, we compare the specific structural and biological properties of distinct soluble prefibrillar alpha-syn oligomers formed either spontaneously or in the presence of dopamine and glutaraldehyde. We show that both on-fibrillar assembly pathway and distinct dopamine-mediated and glutaraldehyde-cross-linked alpha-syn oligomers are only slightly effective in perturbing cell membrane integrity and inducing cytotoxicity, while mature fibrils exhibit the highest toxicity. In contrast to low-molecular weight and unstable oligomers, large stable alpha-syn oligomers seed the aggregation of soluble alpha-syn within reporter cells although to a lesser extent than mature alpha-syn fibrils. These oligomers appear elongated in shape. Our findings suggest that alpha-syn oligomers represent a continuum of species ranging from unstable low molecular weight particles to mature fibrils via stable elongated oligomers composed of more than 15 alpha-syn monomers that possess seeding capacity.
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