期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep19792
关键词
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资金
- HarvestPlus
- Global Rice Science Partnership (GRiSP)
- U.S. Agency for International Development (USAID-Development of Rice Biotechnology Products for Asia grant)
More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 mu g g(-1) iron (Fe) and 16 mu g g(-1) zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 mu g g(-1) Fe and 28 mu g g(-1) Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 mu g g(-1) in glasshouse settings; in contrast, under field conditions, 4 mu g g(-1) was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 mu g g(-1) Fe and 45.7 mu g g(-1) Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.
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