期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/srep19766
关键词
-
资金
- Australian Society for Parasitology Network Researcher Exchange Award
- John & Betty McCreery Traveling Scholarships
- National Health and Medical Research Council of Australia (NHMRC) [APP1047085]
- Wellcome Trust [094277, 100993/Z/13/Z]
- National Health Service (NHS) Blood and Transplant Research and Development programme
- Australian Postgraduate Award
- NHMRC [APP1055246]
- Australian Research Council (ARC) [FT100100112]
The invasive blood-stage malaria parasite-the merozoite-induces rapid morphological changes to the target erythrocyte during entry. However, evidence for active molecular changes in the host cell that accompany merozoite invasion is lacking. Here, we use invasion inhibition assays, erythrocyte resealing and high-definition imaging to explore red cell responses during invasion. We show that although merozoite entry does not involve erythrocyte actin reorganisation, it does require ATP to complete the process. Towards dissecting the ATP requirement, we present an in depth quantitative phospho-proteomic analysis of the erythrocyte during each stage of invasion. Specifically, we demonstrate extensive increased phosphorylation of erythrocyte proteins on merozoite attachment, including modification of the cytoskeletal proteins beta-spectrin and PIEZO1. The association with merozoite contact but not active entry demonstrates that parasite-dependent phosphorylation is mediated by host-cell kinase activity. This provides the first evidence that the erythrocyte is stimulated to respond to early invasion events through molecular changes in its membrane architecture.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据