期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep20400
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资金
- Gansu Province Science Foundation for Distinguished Young Scholars [1308RJDA014]
- Longyuan Support Project for Young Creative Talents [GANZUTONGZI [2014]]
- Technology Program of Gansu Province [1205TCYA034]
- Technology Program of Lanzhou City [2013-4-115, 2015-3-93]
- Fundamental Research Funds for the Central Universities of China [lzujbky-2015-57]
- Natural Science Foundation of Gansu Province [1208RJZA219]
- Program of Science and Technology Innovation Teams Building in Heilongjiang Province [2012TD006]
- Scientific Research Fund of Heilongjiang Provincial Education Department [12511361]
Bacillus subtilis strain B7-S screened from18 strains is an aerobic, endospore-forming, model organism of Gram-positive bacteria which is capable to form vanillin during ferulic acid bioconversion. The bioconversion of ferulic acid to vanillin by Bacillus subtilis B7-S (B. subtilis B7-S) was investigated. Based on our results, the optimum bioconversion conditions for the production of vanillin by B. subtilis B7-S can be summarized as follows: temperature 35 degrees C; initial pH 9.0; inoculum volume 5%; ferulic acid concentration 0.6 g/L; volume of culture medium 20%; and shaking speed 200 r/min. Under these conditions, several repeated small-scale batch experiments showed that the maximum conversion efficiency was 63.30% after 3 h of bioconversion. The vanillin products were confirmed by spectral data achieved from UV-vis, inductively coupled plasma atomic emission spectroscope (ICP-AES) and Fourier transform infrared spectrometer (FT-IR) spectra. Scanning electron microscopy (SEM) and transmission electron spectroscopy (TEM) results confirmed that the cell surface of B. subtilis plays a role in the induction of ferulic acid tolerance. These results demonstrate that B. subtilis B7-S has the potential for use in vanillin production through bioconversion of ferulic acid.
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