期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep22252
关键词
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资金
- Precursory Research for Embryonic Science and Technology program of the Japanese Science and Technology Agency
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [22228006]
- Grants-in-Aid for Scientific Research [22228006] Funding Source: KAKEN
Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.
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