期刊
SCIENTIFIC REPORTS
卷 4, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep04957
关键词
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资金
- Japan Society for the Promotion of Science
- Innovative Areas Glia Assembly of The Ministry of Education, Culture, Sports, Science, and Technology, Japan [25117011]
- Health and Labour Sciences Research [H24-Seishin-Jitsuyouka (Seishin)-Ippan-001]
- Young Principal Investigators' Research Grant of Innovation Center for Medical Redox Navigation, Kyushu University
- Takeda Science Foundation - Medical Research
- SENSHIN Medical Research Foundation
- Grants-in-Aid for Scientific Research [24650227, 26713039, 26860933, 25117011, 25117001, 25253005] Funding Source: KAKEN
Microglia have been implicated in various neurological and psychiatric disorders in rodent and human postmortem studies. However, the dynamic actions of microglia in the living human brain have not been clarified due to a lack of studies dealing with in situ microglia. Herein, we present a novel technique for developing induced microglia-like (iMG) cells from human peripheral blood cells. An optimized cocktail of cytokines, GM-CSF and IL-34, converted human monocytes into iMG cells within 14 days. The iMG cells have microglial characterizations; expressing markers, forming a ramified morphology, and phagocytic activity with various cytokine releases. To confirm clinical utilities, we developed iMG cells from a patient of Nasu-Hakola disease (NHD), which is suggested to be directly caused by microglial dysfunction, and observed that these cells from NHD express delayed but stronger inflammatory responses compared with those from the healthy control. Altogether, the iMG-technique promises to elucidate unresolved aspects of human microglia in various brain disorders.
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