期刊
SCIENTIFIC REPORTS
卷 3, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep03072
关键词
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资金
- Underwood Trust Endowment Program grant
- Dunhill Medical Trust [R128/1109]
- NIHR Biomedical Research Centre at Moorfields Eye Hospital
- UCL Institute of Ophthalmology
- NIHR Research Professorship
- National Institute for Health Research [NF-SI-0508-10130, NIHR-RP-011-003] Funding Source: researchfish
Macrophages are rapidly conditioned by cognate and soluble signals to acquire phenotypes that deliver specific functions during inflammation, wound healing and angiogenesis. Whether inhibitory CD200R signaling regulates pro-angiogenic macrophage phenotypes with the potential to suppress ocular neovascularization is unknown. CD200R-deficient bone marrow derived macrophages (BMM Phi) were used to demonstrate that macrophages lacking this inhibitory receptor exhibit enhanced levels of Vegfa, Arg-1 and Il-1 beta when stimulated with PGE(2) or RPE-conditioned (PGE(2)-enriched) media. Endothelial tube formation in HUVECs was increased when co-cultured with PGE(2)-conditioned CD200R(-/-) BMM Phi, and laser-induced choroidal neovascularization was enhanced in CD200R-deficient mice. In corroboration, signaling through CD200R results in the down-regulation of BMM Phi angiogenic and pro-inflammatory phenotypes. Translational potential of this pathway was investigated in the laser-induced model of choroidal neovascularization. Local delivery of a CD200R agonist mAb to target myeloid infiltrate alters macrophage phenotype and inhibits pro-angiogenic gene expression, which suppresses pathological angiogenesis and CNV development.
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