期刊
SCIENTIFIC REPORTS
卷 3, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep02866
关键词
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资金
- KAKENHI [24228008]
- Program for Promotion of Basic and Applied Researchers for Innovations in Bio-oriented Industry (BRAIN)
- Scottish Government
- BBSRC [BB/J00247X/1, BB/H002286/1]
- RIKEN Foreign Postdoctoral Researcher (FPR)
- CSIRO OCE (Office of the Chief Executive) postdoctoral fellowships
- Grants-in-Aid for Scientific Research [22119010, 24770053] Funding Source: KAKEN
- BBSRC [BB/H002286/1, BB/J00247X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H002286/1, BB/J00247X/1] Funding Source: researchfish
RNA-binding proteins (RBPs) play an important role in plant host-microbe interactions. In this study, we show that the plant RBP known as FPA, which regulates 3'-end mRNA polyadenylation, negatively regulates basal resistance to bacterial pathogen Pseudomonas syringae in Arabidopsis. A custom microarray analysis reveals that flg22, a peptide derived from bacterial flagellins, induces expression of alternatively polyadenylated isoforms of mRNA encoding the defence-related transcriptional repressor ETHYLENE RESPONSE FACTOR 4 (ERF4), which is regulated by FPA. Flg22 induces expression of a novel isoform of ERF4 that lacks the ERF-associated amphiphilic repression (EAR) motif, while FPA inhibits this induction. The EAR-lacking isoform of ERF4 acts as a transcriptional activator in vivo and suppresses the flg22-dependent reactive oxygen species burst. We propose that FPA controls use of proximal polyadenylation sites of ERF4, which quantitatively limit the defence response output.
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