期刊
SCIENTIFIC REPORTS
卷 3, 期 -, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/srep03194
关键词
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资金
- Grains Research and Development Corporation
- Australian Research Council
- South Australian Government
- University of Adelaide
- Australian Grain Technologies
- CSIRO Ecosystem Sciences
- South Australian Grain Industry Trust Fund
- European Community [FP7-244374]
Root systems are critical for water and nutrient acquisition by crops. Current methods measuring root biomass and length are slow and labour-intensive for studying root responses to environmental stresses in the field. Here, we report the development of a method that measures changes in the root DNA concentration in soil and detects root responses to drought in controlled environment and field trials. To allow comparison of soil DNA concentrations from different wheat genotypes, we also developed a procedure for correcting genotypic differences in the copy number of the target DNA sequence. The new method eliminates the need for separation of roots from soil and permits large-scale phenotyping of root responses to drought or other environmental and disease stresses in the field.
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