期刊
SCIENTIFIC REPORTS
卷 3, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep02308
关键词
-
资金
- National Natural Science Foundation of China [21077063, 21277173]
- National Instrument Major Project of China [2012YQ3011105]
- Basic Research funds in Renmin University of China from the central government [13XNLJ01]
A structure-switching DNA optical biosensor for rapid on-site/in situ detection of heavy metal ions is reported. Mercury ions (Hg2+), highly toxic and ubiquitous pollutants, were selected as model target. In this system, fluorescence-labeled DNA containing T-T mismatch structure was introduced to bind with DNA probes immobilized onto the sensor surface. In the presence of Hg2+, some of the fluorescence-labeled DNAs bind with Hg2+ to form T-Hg2+-T complexes through the folding of themselves into a hairpin structure and dehybridization from the sensor surface, which leads to decrease in fluorescence signal. The total analysis time for a single sample was less than 10 min with detection limit of 1.2 nM. The rapid on-site/in situ determination of Hg2+ was readily performed in natural water. This sensing strategy can be extended in principle to other metal ions by substituting the T-Hg2+-T complexes with other specificity structures that selectively bind to other analytes.
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