期刊
SCIENTIFIC REPORTS
卷 2, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep00306
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资金
- Region Ile-de-France (DIM-Malinf)
- Agence Nationale de la Recherche [ANR-07-BLAN-0263]
- Agence Nationale de la Recherche (ANR) [ANR-07-BLAN-0263] Funding Source: Agence Nationale de la Recherche (ANR)
We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump.
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