期刊
SCIENTIFIC REPORTS
卷 1, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep00135
关键词
-
资金
- Spanish Ministerio de Ciencia e Innovacion, DIMEGEN [BIO2008-02444, CSD2009-00006, BIO2009-10138, CGL2010-19303]
- Generalitat Valenciana [PROMETEO/2010/089]
- Junta de Andalucia [P10-CVI-6226]
- NSF [DEB-919290]
- FEDER
- Junta de Andalucia
- Slovenian Research Agency [P1-0198]
- U.S. National Science Foundation [DEB-841933, OCE-821374]
- Direct For Biological Sciences
- Division Of Environmental Biology [0830024, 0841933] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Division Of Environmental Biology [919290] Funding Source: National Science Foundation
We describe the microbiota of two hypersaline saltern ponds, one of intermediate salinity (19%) and a NaCl saturated crystallizer pond (37%) using pyrosequencing. The analyses of these metagenomes (nearly 784 Mb) reaffirmed the vast dominance of Haloquadratum walsbyi but also revealed novel, abundant and previously unsuspected microbial groups. We describe for the first time, a group of low GC Actinobacteria, related to freshwater Actinobacteria, abundant in low and intermediate salinities. Metagenomic assembly revealed three new abundant microbes: a low-GC euryarchaeon with the lowest GC content described for any euryarchaeon, a high-GC euryarchaeon and a gammaproteobacterium related to Alkalilimnicola and Nitrococcus. Multiple displacement amplification and sequencing of the genome from a single archaeal cell of the new low GC euryarchaeon suggest a photoheterotrophic and polysaccharide-degrading lifestyle and its relatedness to the recently described lineage of Nanohaloarchaea. These discoveries reveal the combined power of an unbiased metagenomic and single cell genomic approach.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据