期刊
SCIENCE IN CHINA SERIES B-CHEMISTRY
卷 52, 期 9, 页码 1504-1511出版社
SCIENCE PRESS
DOI: 10.1007/s11426-009-0206-9
关键词
ruthenium(II) complex; DNA binding; DNA photocleavage; PDT
资金
- 973 Program of China [2007CB815306]
- National Natural Science Foundation of China [20771105, 20871122]
- New Century Excellent Talents in University [NCET-06-0718]
- Ministry of Education [108103]
- Natural Science Foundation of Guangclong Province [9351027501000003]
Two new ruthenium(II) complexes, [Ru(btz)(3)](ClO4)(2) (1) and [Ru(btz)(dppz)(2)](ClO4)(2) (2) (btz = 4,4'-bithiazole, dppz = dipyrido[3,2-a:2',3'-c]phenazine), have been synthesized and characterized by elemental analysis, H-1 NMR, ES-MS and X-ray crystallography. The DNA binding behaviors of two complexes have been studied by spectroscopic and viscosity measurements. The results suggest that complex 1 binds to CT-DNA via an electrostatic mode, while complex 2 via an intercalative mode. Under irradiation at 365 nm, both complexes were found to promote the cleavage of plasmid pBR 322 DNA from supercoiled form I to nicked form II. The mechanism studies reveal that singlet oxygen O-1(2) and hydroxyl radical (OH.) play a significant role in the photocleavage process.
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