期刊
RSC ADVANCES
卷 8, 期 51, 页码 29385-29391出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8ra04953j
关键词
-
资金
- National Natural Science Foundation of China [21375009, U1530141]
Due to the indiscriminate usage of organophosphates in military and agriculture, there is an increasing demand to develop sensors for monitoring organophosphorus nerve agents and pesticides. Herein, a sensitive two-dimensional photonic crystal (2D-PC) biosensor as a part of a portable device for the detection of Dipterex is presented. The 2D-PC array was self-organized on the water-air interface using 600 nm polystyrene (PS) colloidal particles, and it was further embedded into a polyacrylamide-acrylic acid (PAM-AA) hydrogel. After condensation between the amine groups of acetylcholinesterase (AChE) and the carboxyl groups of the hydrogel, a novel sensing platform of 2D-PC hydrogel with AChE as the recognition agent was constructed. The lattice spacing of AChE-functionalized 2D-PC was inversely proportional to the logarithm of the Dipterex concentration from 10(-14) mol L-1 to 10(-4) mol L-1 with estimated limit of detection (LOD) of 7.7 x 10(-15) mol L-1. Simultaneously, the structural color of AChE-functionalized 2D-PC varied from yellow to blue. The biosensor achieved naked-eye detection due to which the use of AChE-functionalized 2D-PC is a promising technique for onsite and fast screening of organophosphates.
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