期刊
ACS PHOTONICS
卷 2, 期 8, 页码 1099-1107出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsphotonics.5b00152
关键词
plasmonics; nanoantenna; fluorescence enhancement; fluorescence correlation spectroscopy; gold nanoparticle; self-assembly
类别
资金
- European Commission [FP7-ICT-2011-7, 288263]
- ERC [StG 278242]
- French Agence Nationale de la Recherche [ANR-11-BS10-002-02]
- Erasmus Mundus Doctorate Program Europhotonics (MUNDUS-EMJD) [159224-1-2009-1-FR-ERA]
Plasmonic antennas offer extremely promising strategies to enhance single molecule fluorescence sensing and breach the limitations set by diffraction. However, the technical difficulty and limited availability of top-down nanofabrication techniques enabling nanometer gap sizes are limiting the impact of plasmonic antennas for biochemical and biophysical applications. Here we demonstrate the effectiveness of self-assembled nanoparticle gap antennas to enhance single molecule fluorescence detection at high concentrations. For a dimer of 80 nm gold nanoparticles with 6 nm gap, we isolate detection volumes down to 70 zL (equivalent to lambda(3)/3600) and achieve 600-fold fluorescence enhancement, microsecond transit time, and operation of fluorescence correlation spectroscopy at concentrations exceeding 10 mu M. We quantify the near-field detection volume and the fluorescence enhancement for different self-assembled nanoantenna designs using fluorescence correlation spectroscopy. The combination of the fabrication simplicity with the large fluorescence enhancement makes the self-assembled colloidal nanoparticle gap antennas optimal to extend a wide variety of single-molecule applications toward the biologically relevant micromolar concentration regime.
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