期刊
RSC ADVANCES
卷 4, 期 74, 页码 39140-39144出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra06296e
关键词
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资金
- Converging Research Center Program [2011K000864]
- National Research Foundation of Korea - Ministry of Science, ICT and Future Planning [NRF-2013R1A2A1A05006378]
- Rural Development Administration, Korea [PJ009842]
This paper demonstrates an inertia-activated cell sorting method to separate cells based on their surface protein expression by using inertial microfluidics. Target cells are immune-specifically reacted with antibody-coated microbeads and then separated from nontarget cells. As a proof of concept, separation of MCF-7 breast cancer cells from U937 lymphoma cells was achieved with 97.6% target cell recovery rate, 95% nontarget cell rejection ratio, 73.8% purity, and an enrichment ratio of 93 at a total flow rate of 8.75 mL h(-1) without using any external forces.
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