期刊
RSC ADVANCES
卷 4, 期 108, 页码 63533-63538出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra13780a
关键词
-
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [18001001, 22000007]
- Japan Society for the Promotion of Science for Young Scientists
A fluorescent probe to detect specific sequences in double-stranded DNA (dsDNA) has been synthesized by conjugating thiazole orange (TO) with pseudo-complementary peptide nucleic acid (pcPNA). A pair of TO-conjugated pcPNAs bound to the target site in dsDNA through double-duplex invasion, and promoted intercalation of the TO moieties for notable enhancement of their fluorescence. Site-specificity of the detection is high enough to differentiate only one base-pair difference in DNA. These TO-pcPNA conjugates also function as site-selective photo-damage inducers. Upon irradiation with visible light, singlet oxygen was generated by energy transfer from the dyes at the invasion site, resulting in the site-selective formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxoG) there.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据