期刊
RSC ADVANCES
卷 4, 期 82, 页码 43624-43629出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra04975f
关键词
-
资金
- National Natural Science Foundation of China [61171020, 21203247, 61376125]
A new graphene-based electrochemical biosensor was constructed and applied for the simple, rapid and highly selective determination of glucose. The modified glassy carbon electrode (GCE) was prepared using dopamine (DA) as a reducing agent for graphene oxide (GO) and as a capping agent to stabilize and coat the resulting reduced graphene oxide (RGO) surface as it can polymerize to form polydopamine (PDA). PDA-RGO composites were dropped onto a glassy carbon electrode surface, and glucose oxidase (GOx) was immobilized on the surface of the composites by Michael addition. Finally, Pt nanoparticles (PtNPs) were electrodeposited on the modified electrode. Such modified electrodes (denoted as GCE/PDA-RGO/GOx/PtNPs) were characterized by UV-visible spectroscopy, atomic force microscopy, X-ray power diffractometry, Fourier-transform infrared spectroscopy, and scanning electron microscopy. Electrochemical behaviors were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The fabricated biosensor exhibited a high sensitivity of 33 mu A mM(-1) cm(-2) over a wide linear range of 0.2 mM to 1 mM, good stability, excellent repeatability, and a detection limit of 0.10 mM.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据