Highly sensitive, stable, and precise detection of dopamine with carbon dots/tyrosinase hybrid as fluorescent probe

A carbon dots/tyrosinase (CDs/TYR) hybrid as a low-cost fluorescent probe for the detection of dopamine exhibits high sensitivity, stability, and precision. The detection limit of dopamine is as low as 6.0 x 10(-8) mol L-1, and the wide detection range is from 1.318 x 10(-4) mol L-1 to 2.06 x 10(-7) mol L-1. This kind of fluorescent probe did not need enzyme immobilization and modification, and the experiment results could be revealed as soon as the probe-sample incubation was completed. More importantly, these test results are comparable to that of the present clinical fluorescence detection and high-performance liquid chromatography (HPLC), and the results show that the three methods agreed well with each other.