期刊
RSC ADVANCES
卷 4, 期 91, 页码 50097-50101出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra09066g
关键词
-
资金
- National Science Foundation of China [21001013]
In this paper, we developed a novel fluorescent probe C359 for highly selective detection of H2S over other relevant biothiols. C359 is designed to contain a thiol-specific cleavable disulfide bond. H2S-mediated the disulfide cleavage and subsequent intramolecular cyclization released the masked 7-hydroxyl coumarin, displaying a remarkable fluorescence enhancement. With the promising features in hand, C359 has been applied to detect the activity of CSE (one of H2S-producing enzyme) and build up an assay for screening CSE inhibitors. We anticipated that the enzyme assay using C359 could provide a powerful methodology for screening more potent and selective enzyme inhibitors.
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