A gelatin based pH responsive composite hydrogel has been synthesised by grafting beta-cyclodextrin (beta-CD) to gelatin (Gel) and crosslinking with oxidised dextran (OX-Dex). This pH sensitive beta-CD grafted Gel crosslinked with OX-Dex (beta-CD-g-Gel/OX-Dex) or composite hydrogel (CHG) was used to investigate the colon delivery of 5-fluorouracil (5-FLU), an anti cancer drug. Fourier Transform Infrared Spectroscopy (FTIR) was used to confirm the grafting of beta-CD, crosslinking and drug encapsulation. Powder X-ray diffraction (PXRD) reveals the amorphous character of CHG. Factors affecting the swelling were thoroughly studied. The grafting of beta-CD enhanced the drug encapsulation capacity. Release data were fitted to empirical equations to understand the nature of the drug release profiles. The drug release profile followed the Higuchi model. Degradation and swelling of the hydrogel were found to contribute to the release of drug. Release profiles of 5-FLU were studied both at gastric pH (1.2) and at intestinal pH (7.4); the results showed that release is very low at pH 1.2 and high at pH 7.4. An in vitro cytotoxicity study was carried out on human colon cancer cells; the results showed that drug loaded beta-CD-g-Gel/OX-Dex (CHG) showed enhanced cell inhibition compared to the drug alone. Results of the present investigation exemplify the potential of this novel pH switchable composite hydrogel for the controlled and targeted delivery of the anti cancer drug 5-FLU.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据