期刊
RSC ADVANCES
卷 3, 期 34, 页码 14701-14709出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3ra41666f
关键词
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资金
- Natural Science Research Foundation of China [21277058, 51273084, 21175058]
- Natural Science Foundation of Shandong Province, China [ZR2012BZ002, ZR2011EL029]
A novel electrochemiluminescence (ECL) immunosensor based on a prepared 3D paper device was designed for the sensitive detection of a tumor marker in human serum by using CdTe quantum dot coated carbon microspheres (QD@CMs) as bionanolabels. To construct such an ECL immunosensor, gold-silver nanocomposite-functionalized graphene was prepared and used as a sensing platform, which increased the surface area to capture a large number of primary antibodies as well as improving the electronic transmission rate. With the employment of the as-prepared QD@CMs as bionanolabels, a good ECL performance (excellent precision and sensitivity) was exhibited through a sandwich-type immunoassay. To examine the signal amplification of the immunoassay, the analytical properties of various immunosensor platforms and labels were compared in detail. As a proof-of-concept, cancer antigen 125 was used as a model analyte and satisfactory results were obtained. Therefore, the proposed method provides a new promising platform for clinical immunoassays of other biomolecules.
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