期刊
RSC ADVANCES
卷 3, 期 48, 页码 25960-25969出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3ra44847a
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资金
- U.S. Department of Energy through the DOE Great Lakes Bioenergy Research Center (GLBRC) [DE-FC02-07ER64494]
Cellulase represents a major cost for biochemical conversion of lignocellulosic biomass to biofuels. In the present study, in-house production of cellulases from Ammonia Fiber Expansion pretreated corn stover (AFEX-CS) using Trichoderma reesei Rut C-30 was investigated. Seed culture was conducted in a simple medium containing corn steep liquor (CSL) and AFEX-CS hydrolysate. The effects of fermentation medium components (CSL, AFEX-CS, Tween 80 and CaCO3) on cellulase production were investigated and a simple fermentation medium was developed containing 20 g L-1 AFEX-CS and 4.0 g L-1 CaCO3. A cellulase activity of 1.5 FPU per mL was produced in this medium. Addition of 0.5% (v/v) CSL in the fermentation medium further improved cellulase activity (1.9 FPU per mL). Enzymatic hydrolysis of AFEX-CS using in-house produced enzymes generated high sugar yields with high enzyme loading. Proteomics analyses indicated that EGI and CBHII were deficient in the in-house produced enzyme cocktail when compared to the optimal cocktail for hydrolyzing AFEX-CS.
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