期刊
RSC ADVANCES
卷 3, 期 5, 页码 1557-1563出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2ra22844k
关键词
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资金
- Natural Basic Research Program of China (973 program) [2011CB606206, 2012CB619103]
- National Natural Science Foundation of China [31070849, 51133004]
- Program for New Century Excellent Talents in University [NCET-11-0350]
Fe3O4/PMMA composite nanospheres with diameters ranging from 10 to 90 nm and saturation magnetisation varying from 29 to 39 emu g(-1) have been successfully synthesized via a facile mini-emulsion polymerisation. Through simple surface modification, the nanospheres could be effectively used for fast protein adsorption, His-tagged protein separation and low concentration protein enrichment. The maximum adsorbed amount of lysozyme was as high as 478 mg g(-1) near its isoelectric point using the Fe3O4/PMMA composite nanospheres. After enrichment with the nanosphere removed surfactants, the S/N of cytochrome C (cyt C) at a concentration of 0.5 mg L-1 remarkably increased to 68. Additionally, the surface functionalized nanospheres showed highly selective separation of His-tagged glutathione S-transferase (GST) from cell lysate. The results indicate the Fe3O4/PMMA composite nanospheres could be used as a nanoplatform for multimodal protein separation.
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