One-step preparation of giant lipid vesicles with high encapsulation efficiency using an electrospray technique

The one-step preparation of giant lipid vesicles (few micrometers in diameter) was achieved using a coaxial electrospray technique, with no volatile organic solvents. We also succeeded in encapsulating a variety of water-soluble and insoluble substrates (calcein, dextran, microbeads, and cells) in the giant lipid vesicles.