期刊
NUTRIENTS
卷 5, 期 8, 页码 2955-2968出版社
MDPI
DOI: 10.3390/nu5082955
关键词
antioxidant defences; cocoa flavanols; dietary polyphenols; Ins-1E cells; oxidative biomarkers; type 2 diabetes mellitus
资金
- Programa Consolider-Ingenio from Spanish Ministry of Economy and Competitivity [AGL2010-17579, CSD2007-00063]
Diabetes mellitus is associated with reductions in glutathione, supporting the critical role of oxidative stress in its pathogenesis. Antioxidant food components such as flavonoids have a protective role against oxidative stress-induced degenerative and age-related diseases. Flavonoids constitute an important part of the human diet; they can be found in most plant foods, including green tea, grapes or cocoa and possess multiple biological activities. This study investigates the chemo-protective effect of a cocoa phenolic extract (CPE) containing mainly flavonoids against oxidative stress induced by tert-butylhydroperoxide (t-BOOH) on Ins-1E pancreatic beta cells. Cell viability and oxidative status were evaluated. Ins-1E cells treatment with 5-20 mu g/mL CPE for 20 h evoked no cell damage and did not alter ROS production. Addition of 50 mu M t-BOOH for 2 h increased ROS and carbonyl groups content and decreased reduced glutathione level. Pre-treatment of cells with CPE significantly prevented the t-BOOH-induced ROS and carbonyl groups and returned antioxidant defences to adequate levels. Thus, Ins-1E cells treated with CPE showed a remarkable recovery of cell viability damaged by t-BOOH, indicating that integrity of surviving machineries in the CPE-treated cells was notably protected against the oxidative insult.
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