期刊
NUTRIENTS
卷 5, 期 2, 页码 455-467出版社
MDPI
DOI: 10.3390/nu5020455
关键词
wax apple; insulin resistance; glucose metabolism; glycogenesis; glycolysis; FL83B mouse hepatocytes
资金
- National Science Council, the Republic of China (Taiwan) [NSC 97-2313-B-214-003-MY3]
FL83B mouse hepatocytes were treated with tumor necrosis factor-alpha (TNF-alpha) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent D-glucose derivative, was performed, and the metabolism of carbohydrates was evaluated by examining the expression of glycogenesis or glycolysis-related proteins in insulin-resistant hepatocytes. The results show that WAE significantly improves the uptake of glucose and enhances glycogen content in insulin-resistant FL83B mouse hepatocytes. The results from Western blot analysis also reveal that WAE increases the expression of glycogen synthase (GS), hexokinase (HXK), glucose-6-phosphate dehydrogenase (G6PD), phosphofructokinase (PFK) and aldolase in TNF-alpha treated cells, indicating that WAE may ameliorate glucose metabolism by promoting glycogen synthesis and the glycolysis pathways in insulin-resistant FL83B mouse hepatocytes.
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