期刊
NEUROSCIENCE BULLETIN
卷 28, 期 6, 页码 680-692出版社
SPRINGER
DOI: 10.1007/s12264-012-1281-z
关键词
aquaporin 4; gene deficiency; inflammation; cysteinyl leukotriene receptor; microglia; astrocyte; focal cerebral ischemia
资金
- National Natural Science Foundation of China [81273491, 81072618, 30772561, 30873053]
- Natural Science Foundation of Zhejiang Province, China [Y2090069]
- Qianjiang Rencai Research Plan of Zhejiang Province China [2010R10055]
- Fundamental Research Funds for the Central Universities, China [2009QNA7008]
Aquaporin-4 (AQP4), the main water channel protein in the brain, plays a critical role in water homeostasis and brain edema. Here, we investigated its role in the inflammatory responses after focal cerebral ischemia. In AQP4-knockout (KO) and wild-type mice, focal cerebral ischemia was induced by 30 min of middle cerebral arterial occlusion (MCAO). Ischemic neuronal injury and cellular inflammatory responses, as well as the expression and localization of cysteinyl leukotriene CysLT(2) and CysLT(1) receptors, were determined at 24 and 72 h after MCAO. AQP4-KO mice showed more neuronal loss, more severe microglial activation and neutrophil infiltration, but less astrocyte proliferation in the brain after MCAO than wild-type mice. In addition, the protein levels of both CysLT(1) and CysLT(2) receptors were up-regulated in the ischemic brain, and the up-regulation was more pronounced in AQP4-KO mice. The CysLT(1) and CysLT(2) receptors were primarily localized in neurons, microglia and neutrophils; those localized in microglia and neutrophils were enhanced in AQP4-KO mice. AQP4 may play an inhibitory role in postischemic inflammation.
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