Molecular Diversity Analysis of Pretty Interesting Potyviridae ORF (PIPO) Coding Region in Indian Isolates of Sugarcane streak mosaic virus

Sugarcane streak mosaic virus (SCSMV) is a distinct member of Poacevirus in Potyviridae family causing mosaic disease in sugarcane. In this study, we computationally predicted potyvirus specific overlapping ORF, PIPO (similar to 139 amino acid) in P3 cistron of known SCSMV genome in +1 reading frame using MLOGD. The nucleotide variations in P3 and the predicted PIPO were experimentally validated using SCSMV isolates from India. Phylogenetic relatedness of identified sequences (P3, PIPO) reflects strong selection and at least three subpopulations within the strains of SCSMV in India. These finding considerably broaden future investigation of the SCSMV pathogenesis in sugarcane.