期刊
MYCOLOGICAL RESEARCH
卷 112, 期 -, 页码 1049-1055出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mycres.2008.03.004
关键词
ATP; Ammonium uptake; Glucose uptake; Hydrogen peroxide; NADPH; Nutrient uptake rate; Reactive oxygen species
类别
资金
- University of Strathclyde
- FEMS
Oxidative stress events have been shown to be associated with reduced consumption of nutrients in yeasts, but there are very few studies in filamentous fungi. in the present study we investigated the impact of oxidative stress on glucose and ammonia utilization in batch cultures of Aspergillus niger B1-D. The addition of 1 mm H2O2 Significantly reduced both glucose and ammonia uptake rates in these cultures. Associated with the decreased nutrient uptake, the activity of glyceraldehyde-3-phosphate dehydrogenase was greatly reduced; conversely, the activity of glucose-6-phosphate dehydrogenase remained unchanged. During the period of reduced nutrient uptake, the intracellular ATP and NADPH levels decreased while the amount of trehalose increased. The activities of glutamine synthetase and glutamate dehydrogenase, two key enzymes of ammonia assimilation, remained unchanged in response to H2O2 UP to 1 mm, suggesting the decreased ammonia uptake rate noted under such conditions is not due to enzyme inactivation caused by oxidative stress, but may be due to an insufficient supply of ATP and NADPH, which are required for ammonia assimilation. (C) 2008 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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