期刊
MYCOLOGICAL RESEARCH
卷 112, 期 -, 页码 681-688出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mycres.2007.11.008
关键词
ectomycorrhiza; extramatrical mycelia; ingrowth mesh bags; ITS sequence; molecular identification; Tomentellopsis submollis; Tylospora fibrillosa; UNITE
类别
We studied the effect of apatite amendment on ectomycorrhizal (EM) mycelial biomass production and species composition in a phosphorus-poor spruce forest using sand-filled mesh bags. Control and apatite-amended bags were buried in pairs in the lower part of the organic horizon for one growth season. DNA extraction, PCR of the ITS region, cloning and random sequencing were used to examine the fungal species composition in each bag. Sequences were identified by comparison with the UNITE database and GenBank. Our study confirmed previous results that the major fungal ingrowth in mesh bags was of EM origin. On average 13 species were detected in each bag. Tylospora fibrillosa, Amphinema sp., Tomentellopsis submollis, and Xerocomus badius made up almost 80 % of the EM sequences. High biomass was related to increased dominance of specific species. There were no statistically significant differences in biomass production estimated from PLFA 18:2(,)6, 9, or between fungal communities of apatite-amended and control bags estimated from DNA after one growth season. The potential of the mesh bag method in studies of functional diversity of EM mycelia in the field is discussed. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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