期刊
MATERIALS
卷 2, 期 3, 页码 1402-1416出版社
MDPI AG
DOI: 10.3390/ma2031402
关键词
cell adhesion; cell spreading; cytocompatibility of materials; cell/material interactions; scanning electron microscopy; backscattered electron imaging; image analysis
类别
资金
- BMBF [03N8707, 03FPF00016]
The development of new materials for biological application requires in vitro testing of cell/surface interactions. Cell adhesion and spreading are difficult to quantify as most materials are non-transparent and transmission microscopy cannot be used. Contrast in reflection microscopy is rather poor. We propose an alternative method for the automated screening of cell attachment and spreading using backscattered electron imaging of scanning electron microscopy. The enhanced cell contrast permits study of cell/material interactions by little differences between cells and material.
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