期刊
JALA
卷 14, 期 5, 页码 277-284出版社
ELSEVIER INC
DOI: 10.1016/j.jala.2009.04.007
关键词
spore; Bacillus; ultrasound; sonication; sequential injection analysis; automation
资金
- Chemical and Biological Countermeasures Division of the Department of Homeland Security Science and Technology Directorate
- US DOE [DE-AC06-76RLO 1830]
An automated, flow-through ultrasonic lysis module that is capable of disrupting bacterial spores to increase the DNA available for biodetection is described. The system uses a flow-through chamber that allows for direct injection of the sample without the need for a chemical or enzymatic pretreatment step to disrupt the spore coat before lysis. Lysis of Bacillus subtilis spores, a benign simulant of Bacillus anthracis, is achieved by flowing the sample through a tube whose axis is parallel to the faces of two transducers that deliver 10 W cm(-2) to the surface of the tube at 1.4-MHz frequency. Increases in amplifiable DNA were assessed by real-time PCR analysis that showed at least a 25-fold increase in amplifiable DNA after ultrasonic treatment with glass beads, compared with controls with no ultrasonic power applied. The ultrasonic system and integrated fluidics are designed as a module that could be incorporated into multistep, automated sample treatment and detection systems for pathogens. (JALA 2009;14:277-84)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据