期刊
JOURNAL OF FORENSIC AND LEGAL MEDICINE
卷 20, 期 5, 页码 513-519出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jflm.2013.02.003
关键词
Nullomers; Nullomer-tagged; Internal amplification controls (IAC); Internal positive controls (IPC); DNA barcoding; Contamination; Forensic DNA; STR; Y-STR
资金
- US Department of Defense, Defense Threat Reduction Agency, DNA Safeguard [W81XWH-07-1-000]
Unintended transfer of biological material containing DNA is a concern to all laboratories conducting PCR analysis. While forensic laboratories have protocols in place to reduce the possibility of contaminating casework samples, there is no way to detect when a reference sample is mislabeled as evidence, or contaminates a forensic sample. Thus there is public concern regarding the safeguarding of DNA submitted to crime labs. We demonstrate a method of introducing an internal amplification control to reference samples, in the form of a nullomer barcode which is based upon sequences absent or rare from publically accessible DNA databases. The detection of this barcode would indicate that the source of analyzed DNA was from a reference sample provided by an individual, and not from an evidence sample. We demonstrate that the nullomers can be added directly to collection devices (FTA paper) to allow tagging during the process of sample collection. We show that such nullomer oligonucleotides can be added to existing forensic typing and quantification kits, without affecting genotyping or quantification results. Finally, we show that even when diluted a million-fold and spilled on a knife, the nullomer tags can be clearly detected. These tags support the National Research Council of the National Academy recommendation that Quality control procedures should be designed to identify mistakes, fraud, and bias in forensic science (National Academy of Sciences, 2009). (C) 2013 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.
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