期刊
NUCLEUS
卷 6, 期 4, 页码 326-338出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19491034.2015.1082695
关键词
CNoB; INB; immunoelectron microscopy; nuclear organization; nucleolus; nuclear bodies; p62; SQSTM1; PML bodies; SUMO; ubiquitin
类别
资金
- Centre National de la Recherche Scientifique
- Fondation ARC pour la Recherche
- Agence Nationale pour la Recherche [ANR-14-CE09-0013-01]
- Agence Nationale de la Recherche (ANR) [ANR-14-CE09-0013] Funding Source: Agence Nationale de la Recherche (ANR)
In order to gain insights on the nuclear organization in mammalian cells, we characterized ultrastructurally nuclear bodies (NBs) previously described as fluorescent foci. Using high resolution immunoelectron microscopy (I-EM), we provide evidence that CNoBs (CRM1-Nucleolar bodies) and INBs (Intranucleolar bodies) are distinct genuine nucleolar structures in untreated HeLa cells. INBs are fibrillar and concentrate the post-translational modifiers SUMO1 and SUMO-2/3 as strongly as PML bodies. In contrast, the smallest CRM1-labeled CNoBs are vitreous, preferentially located at the periphery of the nucleolus and, intricately linked to the chromatin network. Upon blockage of the CRM1-dependent nuclear export by leptomycin B (LMB), CNoBs disappear while p62/SQSTM1-containing fibrillar nuclear bodies are induced. These p62 bodies are enriched in ubiquitinated proteins. They progressively associate with PML bodies to form hybrid bodies of which PML decorates the periphery while p62/SQSTM1 is centrally-located. Our study is expanding the repertoire of nuclear bodies; revealing a previously unrecognized composite nucleolar landscape and a new mode of interactions between ubiquitous (PML) and stress-induced (p62) nuclear bodies, resulting in the formation of hybrid bodies.
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